Ammonia Channel Couples Glutaminase with Transamidase Reactions in GatCAB
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概要
- 論文の詳細を見る
The formation of glutaminyl. transfer RNA (Gln-tRNA(Gln)) differs among the three domains of life. Most bacteria employ an indirect pathway to produce Gln-tRNA(Gln) by a heterotrimeric glutamine amidotransferase CAB (GatCAB) that acts on the misacylated Glu-tRNA(Gln). Here, we describe a series of crystal structures of intact GatCAB from Staphylococcus aureus in the apo form and in the complexes with glutamine, asparagine, Mn2+, and adenosine triphosphate analog. Two identified catalytic centers for the glutaminase and transamidase reactions are markedly distant but connected by a hydrophilic ammonia channel 30 angstrom in length. Further, we show that the first U-A base pair in the acceptor stem and the D loop of tRNA(Gln) serve as identity elements essential for discrimination by GatCAB and propose a complete model for the overall concerted reactions to synthesize Gin-tRNA(Gln).
- American Association for the Advancement of Scienceの論文
- 2006-06-30
著者
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Yao Min
Faculty Of Adv. Life Sci. Hokkaido Univ.
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Sakai Naoki
Faculty Of Advanced Life Sciences Hokkaido University
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Chimnaronk Sarin
Faculty of Advanced Life Sciences, Hokkaido University
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Chimnaronk Sarin
Faculty Of Advanced Life Sciences Hokkaido University
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Tanaka Isao
Graduate School Of Life Science Hokkaido University
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Yao Min
Graduate School Of Life Science Hokkaido University
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