Antibody neutralization of TGF-β enhances the deterioration of collagen fascicles in a tissue-cultured tendon matrix with ex vivo fibroblast infiltration
スポンサーリンク
概要
- 論文の詳細を見る
A tissue-cultured tendon matrix infiltrated with cultured fibroblasts can be regarded as an ideal tissue-engineered tendon model. To clarify the role of TGF-β in a tissue-cultured tendon matrix during ex vivo cellular infiltration, the present ex vivo study was conducted to test the following hypothesis that antibody neutralization of TGF-β enhances weakening of the collagen fascicles of the patellar tendon matrix in response to ex vivo fibroblast infiltration. In skeletally mature female rabbits, fibroblasts were isolated from the right patellar tendons using an explant culture technique, and the left patellar tendons underwent multiple freeze/thaw treatment with liquid nitrogen to obtain an acellular tendon matrix. Each acellular tendon was placed in a collagen gel containing cultured fibroblasts and then incubated with or without anti-TGF-β1 antibody for 6 weeks. We found that antibody neutralization of TGF-β enhanced the decrease in the tensile strength and tensile modulus of the collagen fascicles of the patellar tendon matrix in response to ex vivo fibroblast infiltration. The present study indicates a possibility that TGF-β may have a role in suppressing the material deterioration of the fascicles in the tendon during ex vivo cellular infiltration.
- Elsevier Ltd.の論文
著者
関連論文
- Anatomic Double-Bundle Anterior Cruciate Ligament Reconstruction
- In Vivo Imaging of Particle-Induced Inflammation and Osteolysis in the Calvariae of NFκB/Luciferase Transgenic Mice
- Bleeding from the bone marrow enhances remodeling of the in situ frozen-thawed anterior cruciate ligament
- Impact of patellofemoral osteoarthritis on long-term outcome of high tibial osteotomy and effects of ventralization of tibial tubercle
- Clinical results of posterior cruciate ligament retaining TKA with alumina ceramic condylar prosthesis : comparison to Co-Cr alloy prosthesis
- Stress shielding of patellar tendon : effect on small-diameter collagen fibrils in a rabbit model
- Anatomical Reconstruction Procedure for the Anteromedial and Posterolateral Bundles of the Anterior Cruciate Ligament
- In vivo effects of partial electrothermal shrinkage on mechanical properties of the anterior cruciate ligament in rabbits
- The effect of cell-based therapy with autologous synovial fibroblasts activated by exogenous TGF-β1 on the in situ frozen-thawed anterior cruciate ligament
- Deficiency of macrophage migration inhibitory factor gene delays healing of the medial collateral ligament : A biomechanical and biological study
- Ex vivo infiltration of fibroblasts into the tendon deteriorates the mechanical properties of tendon fascicles but not those of tendon bundles
- Antibody neutralization of TGF-β enhances the deterioration of collagen fascicles in a tissue-cultured tendon matrix with ex vivo fibroblast infiltration
- Juvenile Blount's disease : bilateral case with asynchronous onset
- In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities
- A Pilot Study of Anatomic Double-Bundle Anterior Cruciate Ligament Reconstruction With Ligament Remnant Tissue Preservation
- Gender-Based Differences in Outcome After Anatomic Double-Bundle Anterior Cruciate Ligament Reconstruction With Hamstring Tendon Autografts