Plasmodium gallinaceumのin vitroにおける鞭毛放出,exflagellation,に及ぼす温度および炭酸ガス圧の影響

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Examination was made on the influence of temperature and carbon dioxide gas pressure upon exflagellation of the microgametocytes of Plasmodium gallinaceum by the in vitro method described by Shute et al. (1966). Petri dishes containing wet blood smears taken from an infected chicken were put in a CO_2 incubator of which the inside temperature and gas phase were adjusted in advance to a certain degree. The dishes weretaken out of the incubator, and the smears were immediately dried and stained with Giemsa, after incubated for different durations. Exflagellating parasites were counted in 100 microscopic fields (×400). Results obtained were: The optimum temperature to induce exflagellation was found to be 25～27℃. Exflagellating parasites could be Observed at 20℃. While, 33℃ was found to inhibit the phenomenon. The gas conditions of 95% air- 5% CO_2 and 80% air-20% CO_2 were not thought to give any influence upon in vitro exflagellation of P. gallinaceum. It was found that exflagellation either in vitro or in vivo (in the mid-gut of Aedes aegypti) would take more than 10 minutes to develop.
1976-03-30