Comparison of the Methods Available for Purification of Brugia pahangi Microfilariae in the Peritoneal Lavage of Jirds (Meriones unguiculatus)

概要

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The pros and cons of the methods available for the separation and purification of Brugia pahangi microfilariae in jirds peritoneal lavage was studied. The methods examined in the present study were agar-enclosing method described by Nogami et al. (1982) and Sephadex PD-10 column method described by Rathaur et al. (1987). Both methods allowed us to recover live microfilariae alone. The purity of microfilarial suspension was satisfactory in both methods; no peritoneal cells, dead microfilariae nor ovum. The PD-10 column method was superior in the percent recovery of microfilariae; 77-89% of microfilariae loaded were recovered by passage on PD-10 column, while 53-74% of microfilariae embedded were recovered by agar-enclosing method. Agar pads used for separation of microfilariae were fragile and frequently disrupted, resulting in the contamination of peritoneal cells in microfilarial suspension. In addition, the PD-10 method was superior in less time consuming and less laborious procedure. PD-10 column can be used repeatedly. Thus the PD-10 column method is, at present, the best available one for separation and purification of microfilariae from peritoneal lavage.
長崎大学熱帯医学研究所の論文
1989-06-30