Pretreatment of Test Sera with Three Kinds of IgG-Adsorbents Did Not Improve Sensitivity in the Indirect ELISA to Detect Anti-Dengue IgM Antibodies
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概要
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Sera from clinical dengue cases in Malaysia were examined to detect IgG- as well as IgM-class anti-dengue antibodies by the indirect ELISA with or without treatment by 3 kinds of IgG-adsorbents. Relative adsorption factor (AF) as determined by the decreased ELISA-OD ratio was 0.42-0.58 for IgG and 0.33-0.39 for IgM, respectively. After IgG-adsorbents treatment, however, the number of anti-dengue IgM-positive serum specimen decreased from 11-20% of the untreated control. The result indicated that pretreatment of test sera with IgG-adsorbents did not improve or even reduced the sensitivity to detect anti-dengue IgM antibodies in the test sera.
- 長崎大学熱帯医学研究所の論文
- 1993-10-20
長崎大学熱帯医学研究所 | 論文
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