バイオトロンで飼育したコガタアカイエカの日本脳炎ウイルスによる実験的感染

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The laboratory colonized mosquitoes (NF strain) of Culex tritaeniorhynchus summorosus were reared in indoor insectary biotron in which the astronomical day length, temperature and humidity were automatically regulated to change by daily cycle and change gradually by 5 days following the changes in the natural astronomical conditions from early August through the end of next May. Adult female mosquitoes which emerged under various conditions were infected with Japanese encephalitis virus by allowing them to bite on 1 day old infected chickens or by feeding the mixture of rabbit blood and virus suspension and kept in controlled conditions of biotron continuously even after infection. At intervals after infection, 4 to 10 mosquitoes were harvested and a half of them was used for assay of the infectivity of the virus. Another half was processed for the samples of fluorescent antibody method and the distribution of viral antigen in mosquitoes was examined. The results obtained were as follows: 1. In preliminary experiment in which infected mosquitoes were kept in conventional incubators (defective of apparatus controlling the various natural conditions but able to keep constant temperatures), it was found that virus growth in infected mosquitoes was prolonged in time with lower temperatures, however, the virus replication still occurred even when the infected mosquitoes were kept at 15℃ throughout the experiment. 2. The virus in mosquitoes which emerged and infected under the environments of before the end of September began to replicate from 3rd or 5th day after infection and reached the maximum titer on 7th or 10th day. The fluorescent viral antigen observed at first in a part of posterior midgut cells and gradually developed to all over the midguts. When the virus infectivity titer reached the maximum, the fluorescent viral antigen was distributed to fatbody cells, salivary gland cells and thoracic ganglion cells. 3. The virus growth in mosquitoes which emerged and infected under the environments of after the middle part of October was observed from 5th or 7th day after infection and the virus titer reached the maximum on 15th day. The virus titer in this case was constantly very much lower that of mosquitoes which emerged and infected in the conditions of before the end of September. The fluorescent viral antigen was observed only in the limitted part of posterior midgut cells throughout of experiment, however, after the environments in biotron were changed to those of March from January, the fluorescent viral antigen was observed to develop to the whole cells of midguts, fatbody cells, thoracic ganglion cells and salivary gland cells.
長崎大学熱帯医学研究所,Institute of Tropical Medicine, Nagasaki Universityの論文

バイオトロンで飼育したコガタアカイエカの日本脳炎ウイルスによる実験的感染

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