Detection of K-ras Codon 12 Mutation in the Portal Blood during Surgery for Pancreatic Carcinoma

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Background and Aim : It is well known that pancreatic adenocarcinomas harber K-ras gene point mutations, which are frequently restricted to codon 12. Thus, the detection of K-ras codon 12 mutations in pancreatic or duodenal juice may did in the diagnosis of pancreatic adenocarcinomas. In the present study, we investigated whether or not portal blood collected during surgery for pancreatic adenocarcinoma contains the K-ras codon 12 mutation. Methods : During radical resection for pancreatic adenocarcinoma, 10ml of portal blood was taken twice ; first immediately after laparotomy, then just before resection. K-ras mutation was analyzed by a polymerase chain reaction using the primars corresponding to mutant alleles. Results : K-ras mutation was identefied in three of nine patients with pancreatic adenocarclinoma immediately before resection, while none of the patients were positive at the time of laparotomy. The K-ras status in the portal bllod did not correlate with microscopic venous or lymphataic invation. Conclusion : K-ras mutations were identified in portal bllod in some patients with pancreatis adenocarcinoma, which indicates that pancreatic cancer cells migrated into portal bllod during resection and that some adjuvant therapy during surgery may be necessary to cure the pancreatic cancer.
Yamaguchi University Graduate School of Medicineの論文