Interleukin-8 Release during Hypoxia-Reoxygenation by Kupffer Cells in Rats with Obstructive Jaundice

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Cytokine release during hypoxia/reoxygenation by Kupffer cells in male Wistar rats with obstructive jaundice was in vitro to investigate the kinetics of interleukin-8 (IL-8) release by Kupffer cells during hypoxia/reoxygenation, and to study the influence of endotoxin during the rexygenation period.The rats were divided into two groups : one underwent bile duct ligation (Group OJ) ; and the other underwent a shamoperation (Group C).Kupffer cells were isolated by collagenase digestion and centrifugal elutriation.After the cells were exposed to hypoxia (95% nitrogen) , they were exposed to reoxygenation (95% oxygen). In addition, they were stimulated with lipopolysacharide (LPS) (0, 1, 10ng/ml). In both groups, the levels of IL-8 were increased in hypoxia/reoxygenation period, and reoxygenation after hypoxia hurther intensified IL-8 production.In the hypoxia period, the IL-8 levels in Group OJ were significantly increased compared with those in Group C. With the LPS challenge, there was no significant difference in IL-8 levels in either group.In concluion, obstructive jaundice induced Kupffer cells activation with increased IL-8 production during hypoxia/reaxygenation.
Yamaguchi University Graduate School of Medicineの論文