Ability of the free radical scavenger MCI-186 to protect the lens from ultraviolet radiation-induced damage

概要

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The antioxidant MCI-186 eliminates radicals in the brain and has been used to treat brain ischemia under the name of Edaravone (Radicut®). Here, we examined its ability to prevent the lens opacity and DNA damage that occur upon UV radiation. For this purpose, we used organ-cultured rat lenses. We examined the effect of co-culturing the lenses with MCI-186 on the degree of UV light-induced lens opacity. We also measured the levels of the reduced form of glutathione (GSH) as well as the amount of DNA damage via the comet assay. When the lenses were exposed to 5 kj/m2 UV radiation, MCI-186 had no significant effect on lens opacity or GSH levels. However, the comet assay revealed MCI-186 slightly but significantly prevented DNA damage. In addition, when the lenses were exposed to 2 kj/m2 UV radiation, a more palliative dose, DNA damage was strongly inhibited by the drug.