Facile immunostaining and labeling of nonadherent cells using a microfluidic device to entrap the cells(METHODS)

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概要

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• We fabricated a microfluidic device for the entrapment of nonadherent cells. Solution exchange was easily performed by introducing the solution into the cell-trapping microchannel. Immunostaining and labeling of the cell membrane of THP-1 cells were demonstrated using this device, which does not require cumbersome repetition of centrifugation and resuspension steps.

• 公益社団法人日本生物工学会の論文

著者

• Okada Takao

  Research Department R&d Division Taki Chemical Co. Ltd.

• Sugiyama Yukihiro

  Research Institute of Biomolecule Metrology Co., Ltd., Tsukuba, Ibaraki 305-0853, Japan

• Okada Takao

  Research Institute of Biomolecule Metrology (RIBM)

• Yamazoe Hironori

  Stress Signal Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

• El Omri

  Research Institute of Biomolecule Metrology (RIBM)

• Hagihara Yoshihisa

  Stress Signal Research Group, Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

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• Facile immunostaining and labeling of nonadherent cells using a microfluidic device to entrap the cells(METHODS)

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