3-9-3 リグノセルロースからのバイオエタノール生産プロセスにおける自立型リグニン系発酵阻害物質吸着体の開発(3-09 エタノール1,Session 3 バイオマス等)
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概要
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We developed a self-sufficient adsorptive removal system of fermentation inhibitors. The adsorbent was made by optimized heating of the residual lignin obtained by microwave pretreatment of Eucalyptus globulus wood and subsequent enzymatic saccharification or SSCF (Simultaneous Saccharification and Co-fermentation) processes. The soluble fraction separated by the microwave pretreatments contained furfural, 5-HMF and lignin degradation products, but the treatments with the adsorbent intensively decreased concentration of the inhibitors without loss of monosaccharide concentration. The selective absorptivity was confirmed using model solutions containing the inhibitors and carbohydrates. To establish in situ detoxified SSCF process, the pretreated biomass was hydrolyzed with cellulolytic enzymes in the presence of the lignin adsorbent. It was found that concentration of the inhibitors intensively decreased but the reducing sugar yields was not affected by the presence of adsorbent. Therefore, one pot SSCF process with Zymomonas mobilis and the adsorbent was examined, and bioethanol was successfully produced at the ethanol concentration over 5 wt%. The new process produces no wastes and needs no purchase materials. The adsorbent is recyclable and the process relieves load of waste water treatments because the organic compounds in the soluble fraction is removed and converted to energy.
- 一般社団法人日本エネルギー学会の論文
- 2013-07-29
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