202 ヒト骨芽細胞核の力学特性評価システムの開発

概要

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Cell nucleus is full of various components, and has complicated and heterogeneous structure. Though most functions of nuclear domains have been well studied, stiffness of nuclear have not been studied. In this study, the experimental system was developed to measure the stiffness of living cellular nucleus in order to verify the inhomogeneity of the cell nucleus. The system is composed of two devices: a sensor which can measure stiffness of micro cell nucleus, a rotatable device which can locate and rotate the nucleus horizontally. The devices are assembled on the stage of an inverted phase contrast microscope. Experimental studies have been carried out by using normal human osteoblast. The method has shown capability to detect difference of stiffness on cell nucleus. The result shows that there is significant difference between nucleolus and other nucleic domains. In addition, it is found that the stiffness difference of cell nucleolus is clearly shown through making stiffness contour map.
一般社団法人日本機械学会の論文
2011-09-05