DNA fragmentation caused by an overdose of Zeocin(METHODS)

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概要

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• DNA-damaging agent Zeocin is frequently used for vector selection in multiple cell types. We found that vector extracted from the Escherichia coli cells overgrown in the presence of Zeocin was generally contaminated with genomic DNA fragments. High dosage or prolonged cultivation should thus be avoided to minimize the contamination.

著者

• Miyazaki Kentaro

  Bioprocess Research Institute, National Institute of Advanced Industrial Science and Technology (AIST):Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo

• Tsukuda Miyuki

  Bioprocess Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

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• DNA fragmentation caused by an overdose of Zeocin(METHODS)

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