401 スパッタリング法を用いたストロンチウムアパタイト薄膜の作製とその細胞応答性(OS5-(1)オーガナイズドセッション《ライフサポートにおける工学技術》)

概要

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The adhesive strength of strontium apatite (SrAp) films and the cell attachment of human osteosarcoma cell line Saos-2 on the films were investigated. SrAp was coated onto substrates using radio frequency sputtering. The coated films were heated or subjected to hydrothermal treatment. The crystallinity and the surface of the films were observed using X-ray diffractometer (XRD) and a scanning electron microscope (SEM), respectively. The adhesive strength was measured using a pull-out test and the cell attachment was carried out with the osteoblast-like cells. From XRD patterns, as-sputtered film at gas pressure of 0.5 Pa was high crystallinity. In pull-out tests, the results indicate that the adhesive strength of the film increased after the hydrothermal method. In the cell attachment measurement, the number of cells and the cell area on the SrAp film treated with the hydrothermal treatment were higher than those on the as-sputtered SrAp film. Furthermore, the number of vinculin-positive focal adhesion plaques on the film was greater than hydroxyapatite coated films.
一般社団法人日本機械学会の論文
2011-08-26