217303 物理刺激負荷可能な再生血管培養装置の開発(OS07 細胞・生体組織3,オーガナイズド・セッション)
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概要
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Tissue engineering is an alternative approach for the preparation of small-diameter (4〜6 mm) vascular grafts due to the potential to control thrombosis, anastomotic cellular hyperplasia and matrix production. It is very important for tissue-engineered blood vessels (TEBVs) to be cultured under mechanical stimulations, so various bioreactors have been developed. However the operability of these bioreactors was not good, because they were perfusion type bioreactors which were composed of many parts, consumed lots of medium and have the high risk of contamination. Our objective is to develop a non-perfusion type bioreactor we can use easily. In this report we present a rotational bioreactor, which can rotate TEBVs in culture medium and applies mechanical stimulations very easily. C3H10T1/2 cells were seeded to tubular biodegradable scaffolds and cultivated in the rotational bioreactor for 72 hours. The results demonstrated that the numbers of dead cells in TEBVs under dynamic flow conditions were lower than static culture controls.
- 一般社団法人日本機械学会の論文
- 2011-03-17