Enhancement of Desiccation Tolerance and Successful Cryopreservation of Rice Cells Pre-cultured with Sucrose-rich Medium

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Desiccation due to drought and freeze stress is a major threat to plants. Several attempts have been made to induce desiccation tolerance and cryopreservation of cultured plant cells and various plant parts. In this study, we successfully optimized the desiccation and cryopreservation conditions of cultured rice cells. Our results indicated that desiccation tolerance can be artificially induced in rice cells and that suitably controlled pretreatment is required for actively growing cultured cells containing abundant free water. We obtained highest survival when the cells were pre-cultured with 0.4 M sucrose for 5 days and desiccated at 70% relative humidity for 18 h. Our results showed that post-preservation culture medium was also important for the viability of desiccated cells. Precultured and desiccated cells could be preserved at 4℃ for 1 month but not at 26℃.
低温生物工学会の論文
2013-10-15