Metabolic engineering of a novel Klebsiella oxytoca strain for enhanced 2,3-butanediol production(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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Fermentative 2,3-butanediol (2,3-BD) production has been receiving increasing interest for its potential as a platform chemical intended for the production of synthetic rubbers, plastics, and solvents. In this study, Klebsiella oxytoca GSC 12206, a 2,3-BD native hyper-producing and nonpathogenic bacterium, was isolated from a cattle farm. Since this isolate produced a significant amount of lactic acid along with 2,3-BD, its mutant deficient in lactic acid formation was constructed by disrupting the ldhA gene which encodes lactate dehydrogenase. The ldhA gene was deleted precisely by using the pKGS plasmid. When compared to the wild-type strain, the mutant deleted with the ldhA gene in glucose fermentation resulted in an increase of 54%, 13%, 60%, and 78% of 2,3-BD titer, productivity, yield, and selectivity, respectively. A fed-batch fermentation by this mutant with intermittent glucose feeding produced 115 g/L of 2,3-BD with an yield and productivity of 0.41 g 2,3-BD per g glucose and 2.27 g/L h, respectively, indicating the usefulness for the industrial production of 2,3-BD.
著者
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Chang Yong
BioSystem Engineering Laboratory, Department of Chemical and Biomolecular Engineering, KAIST
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Kim Duk-Ki
Research and Development Center, GS Caltex Corporation
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Rathnasingh Chelladurai
Research and Development Center, GS Caltex Corporation
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Song Hyohak
Research and Development Center, GS Caltex Corporation
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Lee Hee
Research and Development Center, GS Caltex Corporation
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Seung Doyoung
Research and Development Center, GS Caltex Corporation
関連論文
- Metabolic engineering of a novel Klebsiella oxytoca strain for enhanced 2,3-butanediol production(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- 2,3-Butanediol recovery from fermentation broth by alcohol precipitation and vacuum distillation(BIOCHEMICAL ENGINEERING)