Intact structure of EGAM1 homeoproteins and basic amino acid residues in the common homeodomain of EGAM1 and EGAM1C contribute to their nuclear localization in mouse embryonic stem cells(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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Recently, we identified the structurally related homeoproteins EGAM1, EGAM1N, and EGAM1C in both preimplantation mouse embryos and mouse embryonic stem (ES) cells. These EGAM1 homeoproteins act as positive or negative regulators of differentiation and cell growth in mouse ES cells, such that these proteins are considered transcriptional regulators. In this study, we investigated their nuclear localization and identified the amino acid residues crucial for the nuclear translocation of EGAM1 and EGAM1C. When expressed exogenously in pluripotent ES cells and somatic NIH3T3 cells, all EGAM1 homeoproteins localized to the nucleus. Analysis using the web-based tool PSORTII predicted a potential nuclear localization signal (NLS) motif, RKDLIRSWFITQRHR, in the homeodomain shared by EGAM1 and EGAM1C. The introduction of mutations, such as mutations from K or R, both basic amino acid residues, to A, in this potential NLS resulted in significant impairment of the nuclear localization of both EGAM1 and EGAM1C. In contrast, GFP fusion proteins of all the full-length EGAM1 homeoproteins failed to localize to the nucleus. These results, when taken together, suggest that basic amino acid residues in the common homeodomain of EGAM1 and EGAM1C and the intact structures of the EGAM1 homeoproteins contribute, at least in part, to the nuclear localization of these proteins in mouse ES cells.
- 公益社団法人日本生物工学会の論文
著者
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SATO Sho
Graduate School of Materials Science, Nara Institute of Science and Technology
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Murata Jun
Graduate School Of Biological Sciences Nara Institute Of Science And Techonology
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Kasuga Kano
Graduate School of Bioresource Sciences, Akita Prefectural University
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Morita Sanae
Graduate School of Bioresource Sciences, Akita Prefectural University
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Iha Momoe
Graduate School of Bioresource Sciences, Akita Prefectural University
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Mori Yuki
Graduate School of Bioresource Sciences, Akita Prefectural University
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Sugawara Saiko
Graduate School of Bioresource Sciences, Akita Prefectural University
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Kojima Ikuo
Graduate School of Bioresource Sciences, Akita Prefectural University
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Ozaki Noriaki
Graduate School of Bioresource Sciences, Akita Prefectural University
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Muraguchi Hajime
Graduate School of Bioresource Sciences, Akita Prefectural University
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Okano Keiju
Graduate School of Bioresource Sciences, Akita Prefectural University
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Iwashita Jun
Graduate School of Bioresource Sciences, Akita Prefectural University
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Hosaka Masahiro
Graduate School of Bioresource Sciences, Akita Prefectural University
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Kobayashi Masayuki
Graduate School of Bioresource Sciences, Akita Prefectural University
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Sato Sho
Graduate School of Bioresource Sciences, Akita Prefectural University
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- Intact structure of EGAM1 homeoproteins and basic amino acid residues in the common homeodomain of EGAM1 and EGAM1C contribute to their nuclear localization in mouse embryonic stem cells(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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