Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal.

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概要

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• Chlamydomonas reinhardtii is widely used to study many biological processes including biofuel production. Here, we present a rapid transformation technique for cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to transformation of other industrially useful algae by optimizing the electric conditions.

• 2013-06-00

著者

• Yamano Takashi

  Graduate School Of Biostudies Kyoto University

• Fukuzawa Hideya

  Graduate School Of Biostudies Kyoto University

• Iguchi Hiro

  Graduate School Of Agricultural Sciences Nagoya Univ.

• Fukuzawa Hideya

  Graduate School of Biostudies, Kyoto University:Japan Science and Technology Agency, ALCA

関連論文

• Identification and Expression Analysis of cDNA Encoding a Chloroplast Recombination Protein REC1, the Chloroplast RecA Homologue in Chlamydomonas reinhardtii(Biochemistry & Molecular Biology)
• RECOMBINANT CARBOXYLTRANSFERASE RESPONSIVE TO REDOX, OF PEA PLASTIDIC ACETYL-COA CARBOXYLASE
• Regulatory factor CCM1 is indispensable for low CO_2 stress responses in Chlamydomonas reinhardtii, binds Zinc, and forms a complex in vivo
• Light and Low-CO_2-Dependent LCIB-LCIC Complex Localization in the Chloroplast Supports the Carbon-Concentrating Mechanism in Chlamydomonas reinhardtii
• Functional analysis of LciB, a gene induced by low-CO_2 stress in the green alga Chlamydomonas reinhardtii
• cDNA array analysis of the effect of acetate on photosynthesis in Chlamydomanas reinhardtii
• Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal.

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