Gene expression and function involved in polyol biosynthesis of Trichosporonoides megachiliensis under hyper-osmotic stress(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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概要

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• Among three erythritol reductase isogenes (er1, er2, and er3) in Trichosporonoides megachiliensis SN-124A, er1 and er2 each had one stress response element (STRE) approximately 2 kbp upstream of their respective initiator codon; in contrast, er3 had two STREs, 148 and 40 bp upstream from the initiator codon. Based on intracellular erythritol accumulation and gene expression profiles, er3 seemed to be highly responsive to stress than er1 or er2. Under hyper-osmotic conditions, intracellular glycerol production, increased significantly within 1.5 h together with glycerol-3-phosphate dehydrogenase gene (gpd1) expression; in contrast, neither er gene expression nor the corresponding production of intracellular erythritol increased significantly within the first 1.5 h of hyper-osmotic culture. However, within 24 h of hyper-osmotic culture, erythritol production and er3 gene expression increased significantly and in parallel. Thus, we concluded that, as an initial response to hyper-osmotic growth conditions, T. megachiliensis produces glycerol as an osmoregulatory compatible solute via GPD; however, within 24 h, it begins to produce erythritol, mainly via ER3, as the preferred compatible solute. Heterologous expression of ers in a Saccharomyces cerevisiae mutant indicated that any of three ers might not function in S. cerevisiae for erythritol biosynthesis in spite of ers and corresponding ERs expression. Hence, although er is annotated as a galactose-inducible crystalline-like yeast protein gene (gcy1) homolog, er may be functionally different from gcy1 in glycolytic metabolism. Otherwise, S. cerevisiae is not likely to produce erythrose, the substrate of erythrose reductase due to metabolic characteristics.

• 2013-06-00

著者

• Kasumi Takafumi

  Enzyme Applications Laboratory Division Of Applied Microbiology National Food Research Institute Min

• Koyama Yoshiyuki

  Enzymology And Molecular Biology Lab. Dep. Of Agricultural And Biological Chemistry Nihon Univ.

• Ogihara Jun

  Enzymology and Molecular Biology Laboratory, Department of Chemistry and Life Science, Nihon University

• Kobayashi Yosuke

  Enzymology and Molecular Biology Laboratory, Department of Chemistry and Life Science, Nihon University

• Yoshida Junjiro

  Enzymology and Molecular Biology Laboratory, Department of Chemistry and Life Science, Nihon University

• Iwata Hisashi

  Enzymology and Molecular Biology Laboratory, Department of Chemistry and Life Science, Nihon University

• Kato Jun

  Enzymology and Molecular Biology Laboratory, Department of Chemistry and Life Science, Nihon University

• Kasumi Takafumi

  Enzymology and Molecular Biology Laboratory, Department of Chemistry and Life Science, Nihon University

関連論文

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• Gene expression and function involved in polyol biosynthesis of Trichosporonoides megachiliensis under hyper-osmotic stress(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Cell dispersion culture for the effective growth of Humicola insolens and efficient enzyme production(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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