Purification, characterization, and gene cloning of a cold-adapted thermolysin-like protease from Halobacillus sp. SCSIO 20089(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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Marine sediment is a distinctive habitat of cold enzyme producing bacteria. A protease producing strain Halobacillus sp. SCSIO 20089 was isolated from a marine sediment of South China Sea. Using chromatographic techniques, the extracellular protease was purified to homogeneity from the culture supernatant. The purified protease exhibited maximal activity at 30℃, pH 8.0, and remained more than 20% of its activity at 0℃. Its activation energy was calculated to be 34.4 kJ/mol, suggesting it is a cold-adapted protease. Based on the N-terminal amino acid sequence of the purified enzyme, full gene encoding the enzyme was obtained by combination of degenerate primer PCR and h1TAIL-PCR. The deduced amino acid sequence showed 57% and 52% identity with mesothermal and thermophilic protease in thermolysin family respectively. All these indicate the enzyme is a unique cold-active thermolysin-like protease with potential in both basic research and industrial application areas.
- 2013-06-00
著者
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Li Jie
Key Laboratory Of Isotope Geochronology And Geochemistry Guangzhou Institute Of Geochemistry Chinese
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Zhang Si
Key Laboratory Of Marine Bio-resources Sustainable Utilization Research Center For Marine Microbes Guangdong Key Laboratory Of Marine Materia Medica South China Sea Institute Of Oceanology Chinese Academy Of Sciences
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Yang Jian
Key Laboratory Of Colloid And Interface Chemistry Ministry Of Education Shandong University
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Tian Xinpeng
Key Laboratory Of Marine Bio-resources Sustainable Utilization Research Center For Marine Microbes Guangdong Key Laboratory Of Marine Materia Medica South China Sea Institute Of Oceanology Chinese Academy Of Sciences
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Zhang Si
Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences:Graduate University of the Chinese Academy of Sciences
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Tian Xinpeng
Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences
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Mai Zhimao
Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences
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Yang Jian
Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences:Graduate University of the Chinese Academy of Sciences
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Li Jie
Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences
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Mai Zhimao
Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences:Graduate University of the Chinese Academy of Sciences
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- Purification, characterization, and gene cloning of a cold-adapted thermolysin-like protease from Halobacillus sp. SCSIO 20089(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)