Effects of hanging drop culture conditions on embryoid body formation and neuronal cell differentiation using mouse embryonic stem cells : Optimization of culture conditions for the formation of well-controlled embryoid bodies(CELL AND TISSUE ENGINEERING)
スポンサーリンク
概要
- 論文の詳細を見る
Hanging drop (HD) cultures were carried out with a drop volume of either 20 or 30 μl. An incubation period of 3 days was determined to be appropriate for the formation of well-controlled embryoid bodies (EBs), and the initial cell number was identified as the most critical factor in the growth and neuronal cell differentiation of EBs.
- 2013-05-00
著者
-
Kurosawa Hiroshi
Division Of Medicine And Engineering Science Interdisciplinary Graduate School Of Medicine And Engin
-
Ohnuki Yoshitsugu
Division Of Medicine And Engineering Science Interdisciplinary Graduate School Of Medicine And Engin
-
Kurosawa Hiroshi
Division of Medicine and Engineering Science, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi
関連論文
- Characterization of Embryoid Bodies of Mouse Embryonic Stem Cells Formed under Various Culture Conditions and Estimation of Differentiation Status of Such Bodies(CELL AND TISSUE ENGINEERING)
- Effect of glucose concentration during embryoid body (EB) formation from mouse embryonic stem cells on EB growth and cell differentiation(CELL AND TISSUE ENGINEERING)
- Morphology and Albumin Secretion of Adult Rat Hepatocytes Cultured on a Hydrophobic Porous Expanded Polytetrafluoroethylene Membrane(MEDICAL BIOTECHNOLOGY)
- Effect of Oxygen on In Vitro Differentiation of Mouse Embryonic Stem Cells(CELL AND TISSUE ENGINEERING)
- Development of cystic embryoid bodies with visceral yolk-sac-like structures from mouse embryonic stem cells using low-adherence 96-well plate(CELL AND TISSUE ENGINEERING)
- Effect of glucose concentration during embryoid body (EB) formation from mouse embryonic stem cells on EB growth and cell differentiation
- Methods for Inducing Embryoid Body Formation : In Vitro Differentiation System of Embryonic Stem Cells
- Application of Rho-associated protein kinase (ROCK) inhibitor to human pluripotent stem cells
- Effects of hanging drop culture conditions on embryoid body formation and neuronal cell differentiation using mouse embryonic stem cells : Optimization of culture conditions for the formation of well-controlled embryoid bodies(CELL AND TISSUE ENGINEERING)