Copper ion-stimulated McoA-laccase production and enzyme characterization in Proteus hauseri ZMd44(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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概要

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• The novel bioelectricity-generating bacterium of Proteus hauseri ZMd44 has been first identified to produce McoA-laccase (EC 1.10.3.2) induced by copper sulphate. The optimal concentration of copper is3 mM as supplementation at the beginning of culture or early exponential growth phase, during which laccase is predominantly synthesized. Moreover, the whole cellular and intracellular activities of laccase increase in the degrees of inducible copper concentrations. A possible mechanism for this phenomenon is that copper ions enhance the laccase genetic transcription level during the laccase synthesis thus granting this strain in copper tolerance. McoA-laccase belongs to typical type 1 (T1) Cu site laccase by electron paramagnetic resonance (EPR) analysis of intracellular enzyme. From our results, the optimal temperature and pH are 60℃ and pH 2.2, respectively. The kinetic profiles show that this enzyme is stable under 50℃ and in the slightly acidic environment, making it a potentially useful enzyme in dye decolorization, paper-pulp bleaching and bioremediation industries.

• 公益社団法人日本生物工学会の論文
• 2013-04-00

著者

• Lu Yinghua

  Department Of Chemical And Biochemical Engineering College Of Chemistry And Chemical Engineering Xia

• Ng I-son

  Department Of Chemical And Biochemical Engineering College Of Chemistry And Chemical Engineering Xia

• Zheng Xuesong

  Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University

• Chen Bor-Yann

  Department of Chemical and Materials Engineering, National I-Lan University

• Zheng Xuesong

  Department Of Chemical And Biochemical Engineering College Of Chemistry And Chemical Engineering Xiamen University

• Chen Bor-yann

  Department Of Chemical And Materials Engineering National I-lan University

• Ye Chiming

  Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University

• Ng I-Son

  Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University:The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University

関連論文

• Enhanced Production of Docosahexaenoic Acid Using Schizochytrium sp. by Optimization of Medium Components
• Exploring new strains of dye-decolorizing bacteria(ENVIRONMENTAL BIOTECHNOLOGY)
• Deciphering simultaneous bioelectricity generation and dye decolorization using Proteus hauseri(ENVIRONMENTAL BIOTECHNOLOGY)
• Exploring new strains of dye-decolorizing bacteria
• Copper ion-stimulated McoA-laccase production and enzyme characterization in Proteus hauseri ZMd44(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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