Stable nitrogen isotopic fractionation associated with transamination of glutamic acid to aspartic acid : implications for understanding ^<15>N trophic enrichment in ecological food webs

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概要

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• The stable nitrogen isotope ratio of glutamic acid is increased by 〜8.0‰ during trophic level changes in ecological food webs. It has been hypothesized that significant isotopic fractionation occurs associated with glutamic acid metabolism during transamination or deamination in heterotrophic animals. However, few studies have investigated the isotopic fractionation factor (α) in this process, even though knowledge of this factor is critical in quantitatively evaluating the flux of amino acid metabolism in animals. In this study we determined the value of a associated with an enzymatic transamination of glutamic acid to aspartic acid in vitro. We found 0.1‰ and 2.2‰ enrichment of ^<15>N following the enzymatic transamination of glutamic acid for 10 and 60min, respectively. Using a Rayleigh model, the α value in this reaction was estimated to be 0.9958. Although this value is associated with much uncertainty (0.9949-0.9968) when applying it to test for an 8.0‰ enrichment, 8‰ of ^<15>N-enrichment for a α value of 0.9958 corresponds to 86% of the glutamic acid being reacted during transamination.

• 2012-12-28

著者

• Goto Akiko

  Department Of Chemistry Tokyo Metropolitan University

• Goto Akiko

  Department of Chemistry, Tokyo Metropolitan University:(Present office)Institute of Science and Engineering, Kanazawa University

• Miura Kasumi

  Department of Chemistry, Tokyo Metropolitan University

関連論文

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• Stable nitrogen isotopic fractionation associated with transamination of glutamic acid to aspartic acid : implications for understanding ^N trophic enrichment in ecological food webs
• Fatty Acid Profiles in Relation to Triglyceride Level in the Liver of Dairy Cows
• Stable nitrogen isotopic fractionation associated with transamination of glutamic acid to aspartic acid : implications for understanding ^N trophic enrichment in ecological food webs

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