ヒト白血病細胞を用いたマウス subrenal capsule grafting の検討
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概要
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In this study we improved the prescription of fibrin clot embedded human leukemia cells for murine subrenal capsule grafting. By pretreatment of thrombin, fibrin gel was generated tightly and better suited for grafting and evaluation. Comparison of cell-growth among immunocompetent normal mice, immunosuppressed mice and immunodeficient mice (nude mice) using this assay procedure showed normal mice was out of order for the antitumor evaluation because severe invasion of host immune cells were observed at cell-gtafting site. Otherwise, leukemia cells were well proliferated without host immune response at the subrenal capsule ground in immunosuppressed mice and nude mice. In conclusion nude mice and immunosuppressed mice were right animals for antitumor activity assay by subrenal capsule grafting using fibrin-cancer cell clot.
- 2013-03-31