米糠γ-オリザノールに含まれる主成分24-メチレンシクロアルタニルフェルレートの効率的単離

概要

論文の詳細を見る
γ-Oryzanol is one of the most important natural products from rice bran and exhibits many biological activities. γ-Oryzanol is a mixture of more than 10 similar components and its major components are cycloartenyl ferulate (1) and 24-methylenecycloartanyl ferulate (2). Here we report novel method for efficient isolation of 2 via selective epoxidation of 1 with m-CPBA at 0℃ and open-column chromatography (silica gel) and subsequent several recrystallization (2-PrOH) to afford 7.5 g crude 2 (30% yield, purity 98.3%). Further purification of crude 2 using HPLC gave pure 2 (91% yield, 99.6% purity). Physical data (^1H- and ^<13>C-NMR and MS spectra) of pure 2 were identical with the reported data. Purity of pure 2 was calculated by HPLC analyses using C18, Cholester and C28 packed column.
2012-12-21