Baiting method for estimating the relative inoculum density of the white root rot fungus in soil
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概要
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A baiting method using Lupinus luteus was applied to the relative estimation of the inoculum density of the white root rot fungus, Rosellinia necatrix in soil. Chips (20 g in dry weight) of dormant twigs of Japanese pear colonized by the fungus were serially diluted with 5 L of an orchard soil and incubated for a week. Thirty seeds of L. luteus were sowed at 5 cm intervals into the infested soil, and the plants were grown at 25 ℃ in a greenhouse during a month. Infection on the plants was then checked. Necrotic and/or rotten legions were observed on the hypocotyls and/or tap roots. There was a significant correlation between the dilution rate of the inoculum and the detection frequency of the white root rot fungus in artificially infested soil. This method is considered to be useful for estimating the inoculum density of the white root rot fungus in field soil.
- 日本土壌微生物学会の論文
- 2008-10-30
著者
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Sasaki Atsuko
National Agriculture And Food Research Organization National Institute Of Fruit Tree Science
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YOSHIDA Koji
National Agricultural Research Center for Hokkaido Region
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Shimane Takanori
National Agriculture And Food Research Organization National Institute Of Fruit Tree Science
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Nakamura Hitoshi
National Agriculture And Food Research Organization National Institute Of Fruit Tree Science
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Yoshida Koji
National Agriculture And Food Research Organization National Institute Of Fruit Tree Science
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Nakamura Hitoshi
Plant Pathology Research Team National Institute Of Fruit Tree Science National Agriculture And Food Research Organization
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Shimane Takanori
Plant Pathology Research Team National Institute Of Fruit Tree Science National Agriculture And Food Research Organization
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SASAKI Atsuko
NARO Institute of Fruit Tree Science, National Agriculture and Food Research Organization
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Sasaki Atsuko
Naro Institute Of Fruit Tree Science National Agriculture And Food Research Organization
関連論文
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- Species-specific PCRs differentiate Rosellinia necatrix from R. compacta as the prevalent cause of white root rot in Japan
- Baiting method for estimating the relative inoculum density of the white root rot fungus in soil
- Development of real-time PCR assay using TaqMan probe for detection and quantification of Rosellinia necatrix in plant and soil
- White root rot disease of the lacquer tree Toxicodendron vernicifluum caused by Rosellinia necatrix