Effect of Copper Contamination on Soil Bacterial Communities Analyzed by Length Polymorphism for PCR-Amplified Fragments of 16S Ribosomal DNA

概要

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Soil bacterial community structure was analyzed using model soil systems (microcosms) contaminated with 4 different levels of Cu^<2+>. We used length polymorphism (LPM) of PCR-amplified DNA fragments of 16S ribosomal RNA gene (16S rDNA) to evaluate the diversity of soil bacteria in the model soil systems. PCR (polymerase chain reaction) primers were designed to classify the soil bacteria into three groups as follows: 1) α and δ subclasses of Proteobacteria, 2) β and γ subclasses of Proteobacteria, and 3) Gram-positive bacteria. Bacterial DNA was extracted directly from the soil samples, and LPM patterns for the PCR-amplified DNA fragments were obtained using a high-resolution gel. Considerable changes in the LPM pattern were observed for the β and γ subclasses of Proteobacteria when the Cu^<2+> concentration increased, while minor changes were observed for the other groups. New bands appeared when the Cu^<2+> concentration increased, suggesting that the number of Cu-resistant bacteria species increased, while that of sensitive bacteria decreased with time after the addition of Cu^<2+> to the soil.
日本土壌微生物学会の論文
2004-04-01