Three-Dimensional Observation of Soil Bacteria in Organic Debris with a Confocal Laser Scanning Microscope

スポンサーリンク

概要

• 論文の詳細を見る

• Confocal laser scanning microscopy (CLSM) was applied to three-dimensional (3-D) observation of bacteria harboring in soil organic matters. Ethidium bromide (EB) was employed for staining soil bacteria, since EB did not stain strongly soil constituents other than bacteria. Staining of soil specimens in 100μg/ml of EB at pH 7.2 for 3 min followed by observation under blue excitation offered a satisfactory preparation for CLSM. A sequence of 60 confocal images of a specimen (soil organic debris 18μm thick) was obtained by optical sectioning at different levels in the specimen at intervals of 0.3μm. From the 60 sections, several types of 3-D projections were generated by image processing : a micrograph with a great depth (18μm) of focus, a pair of split stereo images which could be observed stereoscopically with the naked eye, etc. These 3-D images enabled to observe the spatial distribution of bacteria in organic debris in detail including bacterial colonization inside the organic debris. Possible use of CLSM in soil microbiology was discussed.

• 日本土壌微生物学会の論文
• 1995-10-01

著者

• Someya Takashi

  Laboratory Of Microbiology School Of Medical Technology University Of Occupational And Environmental

• Someya T

  Laboratory Of Microbiology School Of Medical Technology University Of Occupational And Environmental Health:(present Office)laboratory Of Soil Microbiology Faculty Of Agriculture Saga University

関連論文

• Three-Dimensional Observation of Soil Bacteria in Organic Debris with a Confocal Laser Scanning Microscope
• Three-Dimensional Observation of Soil Bacteria in Organic Debris with a Confocal Laser Scanning Microscope

スポンサーリンク