Oriented cartilage extracellular matrix-derived scaffold for cartilage tissue engineering(CELL AND TISSUE ENGINEERING)

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概要

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• The structure of a cartilage scaffold is required to mimic native articular cartilage, which has an oriented structure associated with its mechanical function. In this study, an oriented cartilage extracellular matrix (ECM)-derived scaffold was fabricated composed of microtubules arranged in parallel in vertical section. The mechanical property was higher than that of a typical non-oriented scaffold (p<0.05). Oriented and non-oriented scaffolds were seeded with chondrogenic-induced bone mesenchymal stem cells and cell-scaffold constructs were implanted subcutaneously in the dorsa of nude mice. At 4 weeks, all samples stained positive for safranin O, toluidine blue, and collagen type II, but negative for collagen type I. Oriented-structure constructs contained numerous parallel giant bundles of densely packed collagen fibers with chondrocyte-like cells aligned along the fibers. Total DNA, glycosaminoglycans and collagen contents increased with time and these values were similar in the two groups. Compared with the native articular cartilage, the Young's modulus of the tissue-engineered (TE) cartilage reached 42.9%, 23.0% in oriented and non-oriented scaffolds respectively, at 4 weeks. These results indicate that oriented ECM-derived scaffolds enhance the biomechanical property of TE cartilage and thus represent a promising approach to cartilage tissue engineering.

著者

• Duan Chunguang

  Institute Of Orthopaedics Xijing Hospital The Fourth Military Medical University

• Xiong Zhuo

  Department Of Mechanical Engineering Tsinghua University

• Meng Guolin

  Institute Of Orthopaedics Xijing Hospital The Fourth Military Medical University

• Jia Shuaijun

  Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University

• Liu Lie

  Department of Orthopaedics, Baoji Central Hospital

• Pan Weimin

  Department of Human Movement Studies, Xi'an Physical Education University

• Zhang Laquan

  Department of Mechanical Engineering, Tsinghua University

• Liu Jian

  Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University

• Liu Lie

  Department Of Orthopaedics Baoji Central Hospital

• Pan Weimin

  Department Of Human Movement Studies Xi'an Physical Education University

• Zhang Laquan

  Department Of Mechanical Engineering Tsinghua University

• Jia Shuaijun

  Institute Of Orthopaedics Xijing Hospital The Fourth Military Medical University

• Liu Jian

  Institute Of Orthopaedics Xijing Hospital The Fourth Military Medical University

関連論文

• Oriented cartilage extracellular matrix-derived scaffold for cartilage tissue engineering(CELL AND TISSUE ENGINEERING)
• Oriented cartilage extracellular matrix-derived scaffold for cartilage tissue engineering

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