Growth and exopolysaccharide yield of Lactobacillus delbrueckii ssp. bulgaricus DSM 20081 in batch and continuous bioreactor experiments at constant pH(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Some Lactobacillus delbrueckii ssp. bulgaricus strains are able to synthesize exopolysaccharides (EPS) and are therefore highly important for the dairy industry as starter cultures. The aim of this study was to investigate the nutritional requirements for growth and EPS production of Lactobacillus delbrueckii ssp. bulgaricus DSM 20081. A medium was developed from a semi-defined medium (SDM) in which glucose was replaced by lactose and different combinations of supplements (nucleobases, vitamins, salts, sodium formate and orotic acid) were added. Constant pH batch fermentation with the modified medium resulted in an EPS yield of approximately 210 mg glucose equivalents per liter medium. This was a 10-fold increase over flask cultivation of this strain in SDM. Although not affecting cell growth, the mixture of salts enhanced the EPS synthesis. Whereas EPS production was approximately 12 mg/g dry biomass without salt supplementation, a significantly higher yield (approximately 20 mg/g dry biomass) was observed after adding the salt mixture. In continuous fermentation, a maximal EPS concentration was obtained at a dilution rate of 0.3 1/h (80mg EPS/L), which corresponded to a specific EPS production of 49 mg/g dry biomass.
著者
-
Weber Jost
Institute Of Food Technology And Bioprocess Engineering Technische Universitat Dresden
-
Jaros Doris
Institute Of Food Technology And Bioprocess Engineering Technische Universitat Dresden
-
Mende Susann
Institute of Food Technology and Bioprocess Engineering, Technische Universitat Dresden
-
Krzyzanowski Leona
Institute of Food Technology and Bioprocess Engineering, Technische Universitat Dresden
-
Rohm Harald
Institute of Food Technology and Bioprocess Engineering, Technische Universitat Dresden
-
Rohm Harald
Institute Of Food Technology And Bioprocess Engineering Technische Universitat Dresden
-
Mende Susann
Institute Of Food Technology And Bioprocess Engineering Technische Universitat Dresden
-
Krzyzanowski Leona
Institute Of Food Technology And Bioprocess Engineering Technische Universitat Dresden
関連論文
- Improved procedure for nucleus extraction for DNA measurements by flow cytometry of red beet (Beta vulgaris L.) hairy roots(PLANT BIOTECHNOLOGY)
- Growth and exopolysaccharide yield of Lactobacillus delbrueckii ssp. bulgaricus DSM 20081 in batch and continuous bioreactor experiments at constant pH(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)