Effect of membranes with various hydrophobic/hydrophilic properties on lipase immobilized activity and stability(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

スポンサーリンク

概要

• 論文の詳細を見る

• In this study, three membranes: regenerated cellulose (RC), glass fiber (GF) and polyvinylidene fluoride (PVDF), were grafted with 1,4-diaminobutane (DA) and activated with glutaraldehyde (GA) for lipase covalent immobilization. The efficiencies of lipases immobilized on these membranes with different hydrophobic/hydrophilic properties were compared. The lipase immobilized on hydrophobic PVDF-DA-GA membrane exhibited more than an 11-fold increase in activity compared to its immobilization on a hydrophilic RC-DA-GA membrane. The relationship between surface hydrophobicity and immobilized efficiencies was investigated using hydrophobic/hydrophilic GF membranes which were prepared by grafting a different ratio of n-butylamine/1,4-diaminobutane (BA/DA). The immobilized lipase activity on the GF membrane increased with the increased BA/DA ratio. This means that lipase activity was exhibited more on the hydrophobic surface. Moreover, the modified PVDF-DA membrane was grafted with GA, epichlorohydrin (EPI) and cyanuric chloride (CC), respectively. The lipase immobilized on the PVDF-DA-EPI membrane displayed the highest specific activity compared to other membranes. This immobilized lipase exhibited more significant stability on pH, thermal, reuse, and storage than did the free enzyme. The results exhibited that the EPI modified PVDF is a promising support for lipase immobilization.

著者

• Liu Yung-chuan

  Department Of Chemical Engineering National Chung Hsing University

• Yu Chung-cheng

  Department Of Chemical Engineering National Chung Hsing University

• Shieh Chwen-jen

  Biotechnology Center National Chung Hsing University

• Chen Guan-Jie

  Department of Chemical Engineering, National Chung Hsing University

• Kuo Chia-Hung

  Biotechnology Center, National Chung Hsing University

• Chen Chih-I

  Department of Chemical Engineering, Hsiuping Institute of Technology

• Chen Guan-jie

  Department Of Chemical Engineering National Chung Hsing University

• Kuo Chia-hung

  Biotechnology Center National Chung Hsing University

• Chen Chih-i

  Department Of Chemical Engineering Hsiuping Institute Of Technology

関連論文

• Purification of penicillin G acylase using immobilized metal affinity membranes
• Effect of membranes with various hydrophobic/hydrophilic properties on lipase immobilized activity and stability(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Production of D-hydantoinase via surface display and self-cleavage system(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

スポンサーリンク