Growth characteristics, phytate contents, and coagulation properties of soymilk from a low-phytate Japanese soybean (Glycine max (L.) Merr.) line(Plant nutrition)
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概要
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The phytic acid (myo-inositol hexakisphosphate or InsP_6) content of seed crops is important to their nutritional quality. Since it represents 75±10% of the total seed phosphorus (P), phytic acid is also important regarding the management of P in agricultural production. A low-phytate F5 line, No. T-2-250-4-20, was selected from the progeny of a cross between the low-phytate soybean line CX1834 and the Japanese commercial cultivar Tanbakuro. This line and its parents were grown in a field nursery, and the growth characteristics, phytate accumulation, and processing suitability for tofu were evaluated. At full maturity, the weight of seeds per plant of line T-2-250-4-20 was 5.2- and 1.3-fold higher than that of CX1834 and Tanbakuro, respectively. The amount of phytate-phosphorus as a percentage of the total P content in seeds was 23% in line T-2-250-4-20-34, 30% in CX1834, and 69% in Tanbakuro. No significant difference was observed among the three cultivars/lines in their seed magnesium (Mg), potassium (K), crude protein, and sugar. However, the calcium (Ca), crude fat and ash contents in seeds of line T-2-250-4-20-34 and Tanbakuro was lowered compared to that of CX1834. The breaking stress of tofu was estimated employing a rheometer with a decreasing concentration of the coagulant magnesium chloride (MgCl_2), starting at 15.7mmol L^<-1>. In tofu made from Tanbakuro, the concentration of MgCl_2 required to achieve the maximum breaking stress was 12.6mmol L^<-1>; however, it was 9.5mmol L^<-1> for tofu made from T-2-250-4-20-34 and CX1834. The tofu made from Tanbakuro was soft and broke at 6.3mmol L^<-1> MgCl_2, but, in line T-2-250-4-20-34, harder tofu was made with lower MgCl_2 concentrations. No difference was observed among the cultivars/lines in the SDS-PAGE patterns of protein in soymilk. These results indicate that we have developed a low-phytate soybean with adequate productivity, and confirmed that tofu made from the low-phytate T-2-250-4-20-34 soybean becomes coagulated and harder at a lower MgCl_2 concentration than that from high-phytate soybean cultivars.
著者
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Victor Raboy
Usda Ars Small Grains And Potato Germplasm Research Unit
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FUKUDA Yasuko
Graduate School of Biosphere Science, Hiroshima University
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TATSUKAWA Eiko
Graduate School of Biosphere Science, Hiroshima University
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SANEOKA Hirofumi
Laboratory of Plant Nutritional Physiology, Graduate School of Biosphere Science, Hiroshima Universi
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HOSHINA Tohru
Hiroshima Prefectural Technology Research Institute, Agricultural Technology Research Center
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UEFUJI Mitsuhiro
Hiroshima Prefectural Technology Research Institute, Agricultural Technology Research Center
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Saneoka Hirofumi
Laboratory Of Plant Nutritional Physiology Graduate School Of Biosphere Science Hiroshima University
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Tatsukawa Eiko
Graduate School Of Biosphere Science Hiroshima University
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Hoshina Tohru
Hiroshima Prefectural Technology Research Institute Agricultural Technology Research Center
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Uefuji Mitsuhiro
Hiroshima Prefectural Technology Research Institute Agricultural Technology Research Center
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Fukuda Yasuko
Graduate School Of Biosphere Science Hiroshima University