Genetic diversity and spatial distribution of rhizobial bacteria nodulating on black locust (Robinia pseudoacacia) rhizospheres(Soil biology)

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概要

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• Black locust (Robinia pseudoacacia L.) is an economically and ecologically important tree in Japan. The species is widely used for afforestation because of its symbiotic relationship with nitrogen-fixing rhizobia. In this study, genetic diversity of rhizobia isolated from black locust nodules and spatial distribution of their genotypes were examined. From a coastal forest, six black locust saplings including the whole root systems were collected and positions of nodules on the roots were recorded. Bacteria were isolated from each nodule and then genotyped by Polymerase Chain Reaction (PCR)-Restriction Fragment Length Polymorphism (RFLP) and sequencing analysis of the 16S rRNA gene. Dendrogram analysis based on PCR-RFLP and sequencing analysis indicated that Mesorhizobium species dominantly colonized black locust roots in this forest, occupying at least 77% of living nodules. Positions and PCR-RFLP genotypes of the nodules revealed that rhizobia isolated from neighbouring nodules tended to have the same genotype in some cases. Statistical analysis has supported this fact. The clustering distribution of nodules in the same RFLP groups should be considered to contribute to the large genetic diversity of rhizobia.

• 一般社団法人日本土壌肥料学会の論文

著者

• Matsushita Norihisa

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Hogetsu Taizo

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• SHIRAISHI Ayami

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Shiraishi Ayami

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Hogetsu Taizo

  Department Of Forest Science Graduate School Of Agricultural And Life Sciences The University Of Tok

• Hogetsu Taizo

  Laboratory Of Forest Botany Department Of Forest Science Graduate School Of Agricultural And Life Sc

• KOMATSU Masabumi

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Komatsu Masabumi

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

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