30A-B-4 Simple and Rapid Detection of Surface Antigens on Cells with Dielectrophoresis(Dielectrophoretic Approach to Biomedical Applications)

概要

論文の詳細を見る
Simple and rapid detection of surface antigens on cells is possible by immobilization of cells accumulated by positive dielectrophoresis (p-DEP) via effective surface immunoreactions and removal of unbound cells by negative DEP (n-DEP). Cells with the surface antigen accumulated on the band electrode modified with specific antibody during p-DEP generated by the application of AC voltage between the indium tin oxide (ITO) electrode and the band electrode. The removal of accumulated cells to the gap region during n-DEP was used for rapid estimation of the residual cells with a specific surface antigen. We demonstrate here that human promyelocytic leukemia cells with the surface antigen CD33 can be captured on a band electrode modified with anti-CD33 antibody. The time required for the determination of the surface antigen is decreased to 60 s compared to that required by a cell binding assay using microtiter plates (30 min). The present method does not require pretreatment such as target labeling or washing of unbound cells.
2011-10-29