Quantitative Analysis of the Laxative Components in Rhubarb by High Performance Liquid Chromatography

概要

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A quantitative analysis of the laxative components in rhubarb was carried out by high performance liquid chromatography (HPLC). A 70% tetrahydrofuran extract of rhubarb was heated with dilute sulfuric acid at 100℃ to hydrolyze the active glycosides, sennoside and glycosyloxyanthraquinone, to the corresponding aglycones, sennidin and oxyanthraquinone. The hydrolyzate was applied to a Sep-pak C_<18> cartridge and washed with water. The aglycones retained on the cartridge were then eluted with tetrahydrofuran. The eluate was quantitatively analyzed by HPLC. The sennoside content in the rhubarb correlated highly with the laxative activity, whereas the correlation between oxyanthraquinone content and laxative activity was rather low. Con- sequently, it was confirmed that, with great reliability, the laxative activity of a rhubarb can be calculated by an equation based on sennoside content.
日本生薬学会の論文
1985-06-20