Characterization of waves of leukocyte recruitment to the lung allograft and

概要

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MHC-mismatched lung allografts are rapidly rejected by the host immune response. We analyzed cells infiltrating thegrafted lung tissue using a collagenase-digestion method. The grafted lung was filled with host-derived leukocytes as earlyas day 1 post transplantation and the majority of the initial infiltrating cells were granulocytes. This initial influx of granulocyteswaned rapidly, followed by a steady increase in lymphocytes, particularly T cells, and then by macrophages. The proportionof CD4+ T cells that express CD25 were increased in the graft the majority of which were activated CD4+ cells. We appliedcytotoxic T-lymphocyte-associated antigen 4 (CTLA4)-Ig treatment in combination with donor-specific blood transfusion to thetransplantation of lung allograft, which was significantly prolonged by the treatment. To examine the cellular and molecularbasis of the inhibition of the graft rejection, we evaluated number and cytokine mRNA expression of the cells infiltrating in thelung allograft using collagenase-digestion method, although we were unable to detect significant effects of the treatment.Taken together, this study demonstrates that single cell suspensions from cellular infiltrates of lung tissue is useful forphenotypical and functional studies on cells infiltrating lung tissue after graft transplantation.