The 184th residue of β-glucosidase Bgl1B plays an important role in glucose tolerance(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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Bgl1B (ACY09072) is a new GH1 family β-glucosidase derived from a marine microbial metagenomic library. In our previous study, the activity of recombinant Bgl1B was competitively inhibited by glucose with an IC_<50> value of 30mM. Based on amino acids sequence alignment with other GH1 β-glucosidases, including BglB from Paenibacillus polymyxa whose structure has been solved, the 184th and 409th residues of Bgl1B were suggested to be key residues relating to glucose tolerance or other properties. The putative role of the two residues of Bgl1B was investigated by site-directed mutagenesis by replacing His184 and Leu409 with Phe and Glu, respectively. Biochemical characterization data indicated that the mutations had little influence on the enzyme properties. However, in comparison with wild-type Bgl1B, the mutant H184F exhibited lower stability at all tested temperatures and pHs, while L409E exhibited higher stability. Particularly, when using 4-Nitrophenyl-β-D-glucopyranoside (pNPG) as substrate, the mutant H184F exhibited much better glucose tolerance, with a K_i of 76.9mM, than L409E and wild-type Bgl1B (17.2mM and 14.9mM, respectively). These results indicated that the 184th residue might play an important role in the glucose tolerance of Bgl1B.