各種生薬成分に対するモノクローナル抗体の作製と応用

概要

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In the course of a program in developing new enzyme-linked immunosorbent assay (ELISA)-methods for the quantification of bioactive natural products in plants, phytomedicines and animals in a[is and ng scale, monoclonal antibodies against various natural products of medicinal have been developed. The cross-reaction of anti-forskolin antibodies with 7-deacetyl forskolin was 5.6%. A very small cross-reaction appeared with other derivatives. The full measuring range of the assay extends from 5 ngml^<-1> to 5μgml^<-1> A MAb against Δ^1-tetrahydrocannabinolic acid (THCA) was produced. Anti-Δ^1-THCA antibody reacted with a range of other cannabinoids, including many cannabinoids and a spiro-compound, but not with other phenolics. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 (G-Rb1) was produced, which scarcely cross-reacted with ginsenosides Rc and Rd. Immunoaffinity column chromatography using this anti-G-Rb1 MAb appeared to be far superior to previously published separation methods by crystallization and chromatography, and the capacity of the immunoaffinity column as determined by the ELISA was 20μg/ml gel. Anti-glycyrrhizin MAbs were produced. TLC immunostaining, in which TLC-separated glycyrrhetic acid glucuronides were transferred to a polyvinylidene difluoride membrane, converted into a glycyrrhetic acid glucuronide-BSA conjugate, and immunostained, was more sensitive than other staining.
日本生薬学会の論文
2000-06-20