Direct PCR amplification of the 16S rRNA gene from single microbial cells isolated from an Antarctic iceberg using laser microdissection microscopy

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概要

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• Here, we describe a technique that allows the genetic linage analysis of 16S rRNA genes in bacteria observed under a microscope. The technique includes the isolation of microbial cells using a laser microdissection microscope, lysis of the cells, and amplification of the 16S rRNA genes in the isolated cells without interference by bacterial DNA contamination from the experimental environment or reagents. Using this technique, we successfully determined 15 16S rRNA gene sequences in cells isolated from an Antarctic iceberg. These sequences showed 94-100% identity to their closest strains, which included bacteria that occur in aqueous, marine, and soil environments.

著者

• Baba Tomoya

  Transdisciplinary Research Integration Center Research Organization Of Information And Systems 1111

• Yanagihara Katsuhiko

  Transdisciplinary Research Integration Center, Research Organization of Information and Systems, 111

• Niki Hironori

  Transdisciplinary Research Integration Center, Research Organization of Information and Systems, 111

• Niki Hironori

  Transdisciplinary Research Integration Center Research Organization Of Information And Systems 1111

• Yanagihara Katsuhiko

  Transdisciplinary Research Integration Center Research Organization Of Information And Systems 1111

関連論文

• Direct PCR amplification of the 16S rRNA gene from single microbial cells isolated from an Antarctic iceberg using laser microdissection microscopy

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