Demonstration of pneumococcal capsule under immunoelectron microscopy

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概要

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• It is challenging to demonstrate the capsule of Streptococcus pneumoniae (S. pneumoniae) under immunoelectron microscopy because of the thick mucopeptide cell wall hampering proper fixation. A novel rapid freeze fixation method was established to observe the capsule of S. pneumoniae. A strain of serotype 3 of S. pneumoniae isolate was analyzed after rapid freezing. An ethanol freezing-substitution fixing method was applied and immunohistochemical staining with osmium tetroxide was tested. The capsule was confirmed using the serotype 3 specific polyclonal antibodies labeled with colloidal gold particles. To the best of our knowledge, this is the first report of S. pneumoniae capsule by immunoelectron microscope.

著者

• ICHINOSE Akitoyo

  Electron Microscope Laboratory

• Ichinose Akitoyo/

  Electron Microscopy Shop Central Laboratory Institute Of Tropical Medicine Nagasaki University/depar

関連論文

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• Demonstration of pneumococcal capsule under immunoelectron microscopy

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