Preparation of tea catechins using polyamide(METHODS)

概要

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An adsorption separation method using Polyamide-6 (PA) as an adsorbent was developed to separate catechins from green tea extract. The adsorption capacity of total catechins for PA was 193.128mg g^<-1> with an adsorption selectivity coefficient K^B_A of total catechins over caffeine 21.717, which was better than macroporous resin model HPD 600. The Langmuir model and the pseudo-second order mode were primely fitted to describe its equilibrium data and adsorption kinetics, respectively. PA column separation by two-step elution using water and 80% (v/v) aqueous ethanol was established to prepare catechins complex which contained 670.808mg g^<-1> total catechins and 1.828mg g^<-1> caffeine. It is considered that PA was a promising adsorbent for selective isolation of catechins.