New strategy for large-scale preparation of the extracellular domain of tumor-associated antigen HAb18G/CD147 (HAb18GED)(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

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HAb18G/CD147, a cancer-associated biomarker, can promote tumor growth, invasion and metastasis. Here, we established a new strategy to express the extracellular domain of HAb18G/CD147 (HAb18GED) by using the FRT/Flp-In recombinase-based site-directed integration system. Two clones expressing HAb18GED were established, and 600mg HAb18GED was obtained with more than 95% purity. Our results showed that purified HAb18GED exhibited strong activities to stimulate the secretion of pro-MMP2 and MMP-2 and promote the invasion of HCC cells. Overall, our study effectively overcame the difficulty of large-scale HAb18GED preparation and laid a solid foundation for the further studies on structure and functions of HAb18G/CD147.

著者

Li Yu
State Key Laboratory Of Engineering Plastics Institute Of Chemistry Center For Molecular Science The
Zhang Zheng
State Key Laboratory Of Cancer Biology Cell Engineering Research Centre & Department Of Cell Bio
Yang Xiangmin
State Key Laboratory of Cancer Biology, Cell Engineering Research Centre & Department of Cell Biolog
Yang Hushan
Division of Population Science, Department of Medical Oncology, Kimmel Cancer Center, Thomas Jeffers
Yu Xiaoling
State Key Laboratory of Cancer Biology, Cell Engineering Research Centre & Department of Cell Biolog
Xing Jinliang
State Key Laboratory of Cancer Biology, Cell Engineering Research Centre & Department of Cell Biolog
Chen Zhinan
State Key Laboratory of Cancer Biology, Cell Engineering Research Centre & Department of Cell Biolog
Yu Xiaoling
State Key Laboratory Of Cancer Biology Cell Engineering Research Centre & Department Of Cell Bio
Chen Zhinan
State Key Laboratory Of Cancer Biology Cell Engineering Research Centre & Department Of Cell Bio
Yang Hushan
Division Of Population Science Department Of Medical Oncology Kimmel Cancer Center Thomas Jefferson
Yang Xiangmin
State Key Laboratory Of Cancer Biology Cell Engineering Research Centre & Department Of Cell Bio
Xing Jinliang
State Key Laboratory Of Cancer Biology Cell Engineering Research Centre & Department Of Cell Bio
Li Yu
State Key Laboratory Of Cancer Biology Cell Engineering Research Centre & Department Of Cell Bio
Xing Jinliang
State Key Laboratory Of Cancer Biology And Department Of Cell Biology And Cell Engineering Research Center Fourth Military Medical University

New strategy for large-scale preparation of the extracellular domain of tumor-associated antigen HAb18G/CD147 (HAb18GED)(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

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