腎シュウ酸カルシウム結晶の形態学的変化におけるオステオポンチン機能の解明

概要

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An important process in kidney stone formation is the conversion of retentive crystals in renal tubules to concrete stones. Osteopontin (OPN) is the major component of kidney calcium-containing stone matrix. In the present study, we estimated OPN function in ealry morphological changes of calcium oxalate crystals using OPN knockout mice: 100 mg/kg glyoxlate were intraabdominally injected into wild-type mice (WT) abd OPN knockout mice (KO) for a week, and 24hr urine oxalate excretion showed no significant difference between WT and KO. Kidney crystal depositions were clearly detected by Pizzolato staining but not by von Kossa staining in both genotypes, and the number of crystals in KO was significantly fewer than in WT.Morphological observation by polarized light optical microphotography and scanning electron microphotography (SEM) showed large flower-shaped crystals growing in renal tubules in WT, whereas small and uniform crystals in KO. X-ray diffraction detected the crystal components as calcium oxalate monohydrate in both genotypes. Immunohistochemical staining of OPN demonstrated that the WT crystals contained OPN protein but not KO crystals. We concluded that OPN plays a crucial role in the morphological conversion of calcium oxalate crystals to stones in mouse kidneys.
名古屋市立大学の論文
2010-05-06