Evaluation of white-rot fungi-assisted alkaline/oxidative pretreatment of corn straw undergoing enzymatic hydrolysis by cellulase(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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In this study, the effects of biological treatment prior to alkaline/oxidative (A/O) pretreatment using three white-rot fungi (Ganoderma lucidum, Trametes versicolor and Echinodontium taxodii) were evaluated for the enzymatic hydrolysis of corn straw. Among these fungi, Echinodontium taxodii significantly enhanced the efficiency of chemical pretreatment. Subsequent to treatment of corn straw with Echinodontium taxodii for 15 days, the straw was subjected to digestion by 0.0016% NaOH and 3% H_2O_2 at room temperature for 24h, which increased the reducing sugar yield by 50.7%. The hydrolysis model and kinetic parameters were determined from time course data collected throughout the hydrolysis. The initial hydrolysis rate, V_0, of the corn straw increased by 68.5% compared to A/O pretreatment alone, which resulted from an increase in the initial adsorption. The lignin content of the corn straw decreased more significantly after biological and A/O pretreatment than after A/O pretreatment alone. After 72h of enzymatic hydrolysis, the adsorbed cellulase decreased by 24.8% (from 3.67 to 2.76mg ml^<-1>) compared to A/O pretreatment alone. These results indicate that biological treatment improves the desorption of cellulase by enhancing delignification during A/O pretreatment.
社団法人日本生物工学会の論文