Engineering of a novel chimera of superoxide dismutase and Vitreoscilla hemoglobin for rapid detoxification of reactive oxygen species(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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The genes encoding human manganese superoxide dismutase (MnSOD) and Vitreoscilla hemoglobin (VHb) were fused in-frame to generate a bifunctional enzyme that possessed MnSOD and peroxidase-like activities. At neutral pH, the coupling of the SOD and peroxidase reactions revealed that the bifunctional enzyme exhibited a 2.5 times shorter transient period and a 1.67 times higher reaction rate at steady-state conditions. Furthermore, the catalytic rate of the bifunctional enzyme was not affected as much by the external H_2O_2 scavenger catalase. This indicates that the bifunctional protein possesses a greater antioxidant capability, which is possibly due to the close proximity between the active site of MnSOD and the heme moiety of VHb. Our findings not only provide insight into the synergistic functions of SOD and peroxidase but also could potentially be used to develop novel therapeutic agents with more efficient O_2 carrying capability.
著者
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Yainoy Sakda
Department Of Clinical Microbiology Faculty Of Medical Technology Mahidol University
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Isarankura-Na-Ayudhya Chartchalerm
Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University
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Tantimongcolwat Tanawut
Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University
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Bulow Leif
Department of Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund Uni
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Prachayasittikul Virapong
Department of Clinical Microbiology, Faculty of Medical Technology, Mahidol University
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Bulow Leif
Department Of Pure And Applied Biochemistry Center For Chemistry And Chemical Engineering Lund Unive
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Tantimongcolwat Tanawut
Department Of Clinical Microbiology Faculty Of Medical Technology Mahidol University
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Prachayasittikul Virapong
Department Of Clinical Microbiology Faculty Of Medical Technology Mahidol University
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Isarankura-na-ayudhya Chartchalerm
Department Of Clinical Microbiology Faculty Of Medical Technology Mahidol University
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Bulow Leif
Department Of Pure And Applied Biochemistry Center For Chemistry And Chemical Engineering Lund Unive
関連論文
- Engineering of a novel chimera of superoxide dismutase and Vitreoscilla hemoglobin for rapid detoxification of reactive oxygen species(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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