BIO-02 ROTARY DNA-CHIP SCANNER BASED ON A DVD OPTICAL PICK-UP(Bio-medical Equipments I,Technical Program of Oral Presentations)

概要

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We developed a commercial microarray scanning system with two-color fluorescence detection (Cy5 and Cy3) and high speed rotational mechanism, loading 8 typical chip slides simultaneously. The proposed optical scanner based on a modified DVD optical pick-up uses two laser diodes of 650 nm (red) and 532 nm (green) wavelengths for the detection of Cy5 and Cy3 fluorescent dyes, In the scanning system, laser beams are used for both dynamic auto-focusing and exciting Cy5 or Cy3 dye, and PMT detects fluorescent light emitted from DNA markers spotted on a slide glass. We have also designed a novel rotational mechanism loading 8 biochip slides for high speed detection. The real-time focusing actuation and rotational mechanism enables fast scanning response, high accuracy and sensitivity with compact size by simple structure, which are superior to conventional microarray detection systems of the expensive mechanism. The performance of fluorescence biochip scanner is inspected by experiments using various commercial DNA chips such as BAC(bacterial artifical chromosome) oligonucleotide chip, HPV(Human Papiloma Virus) genotyping chip and scanner calibration slide chip(Full moon biosystems), etc.
2009-06-17