Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
This is the first report that Lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. Growth of the E4 Lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53mM ammonium ion concentration) in media containing glucose rather than other tested carbon sources. The optimum glucose concentration was 0.30% at an initial medium pH of 7.0 and incubation temperature of 30℃. From time-course experiments, when the glucose in the culture was exhausted, ammonia began to be accumulated, and maximum ammonia accumulation (〜1.60mM) was reached after 8 days of incubation. Ammonia accumulation by this strain required molybdenum, manganese, and iron.
著者
-
Iwata Kenichi
College of Systems Engineering and Science, Department of Bioscience and Engineering, Shibaura Insti
-
Azlan Azlin
Graduate School of Engineering, Applied Chemistry, Shibaura Institute of Technology
-
Yamakawa Hironori
College of Engineering, Department of Applied Chemistry, Shibaura Institute of Technology
-
Omori Toshio
College of Systems Engineering and Science, Department of Bioscience and Engineering, Shibaura Insti
-
Azlan Azlin
Graduate School Of Engineering Applied Chemistry Shibaura Institute Of Technology
-
Omori Toshio
College Of Systems Engineering And Science Department Of Bioscience And Engineering Shibaura Institu
-
Iwata Kenichi
College Of Systems Engineering And Science Department Of Bioscience And Engineering Shibaura Institu
-
Yamakawa Hironori
College Of Engineering Department Of Applied Chemistry Shibaura Institute Of Technology