N-terminal deletion of Tk1689, a subtilisin-like serine protease from Thermococcus kodakaraensis, copes with its cytotoxicity in Escherichia coli(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

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• Tk1689, a subtilisin-like serine protease from Thermococcus kodakaraensis, was found to be toxic to the host cells when produced in the pro-protein form (Pro-Tk1689) in Eschericia coli. Cytotoxic effect of Pro-Tk1689 was reduced when signal and pro-peptide both were removed and the protein was produced in the mature form (MP-Tk1689). The mature protein was produced in E. coli in the soluble form. Recombinant MP-Tk1689 was catalytically active and exhibited optimum activity at 55℃ and pH 7. Specific activity of the enzyme was 700U/mg. The enzyme displayed a half life of 80min at 60℃.

著者

• Rasool Nouman

  School of Biological Sciences, University of the Punjab

• Rashid Naeem

  School of Biological Sciences, University of the Punjab

• Iftikhar Saima

  School of Biological Sciences, University of the Punjab

• Akhtar Muhammad

  School of Biological Sciences, University of the Punjab

• Rashid Naeem

  School Of Biological Sciences University Of The Punjab

• Rasool Nouman

  School Of Biological Sciences University Of The Punjab

• Iftikhar Saima

  School Of Biological Sciences University Of The Punjab

• Akhtar Muhammad

  School Of Biological Sciences University Of The Punjab:school Of Biological Sciences University Of S

• Akhtar Muhammad

  School Of Biological Sciences University Of The Punjab Quaid-e-azam Campus

• Akhtar Muhammad

  School Of Biological Sciences University Of The Punjab Quaid-e-azam Campus:school Of Biological Scie

関連論文

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