INFLUENCE OF GRAM-NEGATIVE BACTERIUM PSEUDOMONAS FLUORESCENS ON THE PRECIPITATION RATES AND POLYMORPHISM OF CALCIUM CARBONATE MINERALS

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概要

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• Microbes can significantly influence the precipitation processes of calcium carbonate (CaCO_3) minerals by changing the chemical state of the habitats and by interaction of extracellular polymeric substances with mineral surfaces. To evaluate the effects of bacteria on the precipitation rates and polymorphism, we performed precipitation experiments using 100ml batch systems containing the gram-negative bacterium Pseudomonas fluorescens at 25℃ for 10 days. Two types of reaction systems (i.e., with or without Mg^<2+> ions) were prepared: (1) C system; 5.0mM Ca^<2+>, 20mM HCO_3^-, and P. fluorescens (0, 10^5, 10^6, 10^7, 10^8, or 10^9cells/ml), and (2) CM system; 5.0mM Ca^<2+>, 5.0mM Mg^<2+>, 20mM HCO_3^-, and P. fluorescens (0, 10^5, 10^6, 10^7, 10^8, or 10^9cells/ml). Throughout the reaction period, solution pH, concentrations of Ca^<2+>, Mg^<2+>, and HCO_3^- ions, and dissolved proteins and polysaccharides released from P. fluorescens cells were measured daily. The results indicated that P. fluorescens significantly inhibited the precipitation of CaCO_3 minerals, and the precipitation rates in the C and CM systems decreased with increasing bacterial concentrations with the inhibition factors of 0.18 and 0.28, respectively. Furthermore, P. fluorescens inhibited the formation of aragonite and favored calcite formation as the dominant polymorph despite the presence of Mg^<2+> ions in the CM system. These effects of P. fluorescens on the formation processes of CaCO_3 minerals could likely be attributed to the interaction of dissolved extracellular polymeric substances such as proteins, polysaccharide, and DNA with the surface of CaCO_3 minerals. The ability of these substances to interact and possible interaction mechanisms were discussed.

著者

• KAWANO MOTOHARU

  Graduate School of Science and Engineering, Kagoshima University

• HWANG JINYEON

  Division of Earth Environmental System, Pusan National University

• Hwang Jinyeon

  Division Of Earth Environmental System Pusan National University

• Kawano Motoharu

  Graduate School Of Science And Engineering Kagoshima University

• Kawano Motoharu

  Kagoshima Univ. Kagoshima Jpn

関連論文

• INFLUENCE OF GRAM-NEGATIVE BACTERIUM PSEUDOMONAS FLUORESCENS ON THE PRECIPITATION RATES AND POLYMORPHISM OF CALCIUM CARBONATE MINERALS
• BIOCHEMICAL EFFECTS OF THE UNICELLULAR GREEN ALGA CHLORELLA VULGARIS ON THE PRECIPITATION RATES AND POLYMORPHISM OF CALCIUM CARBONATE MINERALS
• EFFECT OF ORGANIC ACIDS ON THE PRECIPITATION RATE AND POLYMORPHISM OF CALCIUM CARBONATE MINERALS IN SOLUTIONS WITH Mg^ IONS

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